Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization.

Preservation of specific RNA distribution within the chromatin-depleted nuclear substructure demonstrated by in situ hybridization coupled with biochemical fractionation

Biochemical fractionation procedures previously shown to remove 95% of cellular protein, DNA, and phospholipid, were combined with fluorescence in situ hybridization to provide a critical evaluation of the retention and spatial preservation of specific primary transcripts within the chromatin-depleted nuclear substructure, operationally defined as the nuclear "matrix." This unique approach made...

Investigation of TEL/AML1 and BCR/ABL Fusion Genes in Patients Affected by Acute Lymphoblastic Leukaemia Using Interphase in situ Hybridization

Development of an Alu-PCR Amplified YAC Probe Suitable for Enumeration of Chromosome 13 on Uncultured Lymphocytes and Amniocytes by Fluorescence in situ Hybridization

The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

Accuracy Assessment of Interphase Fluorescence In-Situ Hybridization on Uncultured Amniotic Fluid Cells

a:4:{s:10:"Background";s:431:"Parental anxiety while waiting for the results of amniocentesis has been investigated by many authors. It seems that the implementation of faster techniques such as fluorescence in-situ hybridization (FISH) will have some benefits in reducing this anxiety. Besides the patients' attitudes to choosing this method, gynecologists who are the persons responsible for tre...

Development of a probe consist of three cosmids to enumerate the chromosome 13 on uncultured lymphocytes or amniocytes using interphase FISH

 Abstract Background: To produce a reliable probe suitable for aneuploidy detection of chromosome 13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization (FISH), we used a contig of three overlapping cosmids mapped to 13q12.3. Methods: The cosmid DNA carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin-11-dUTP. Th...